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Differential effects of agarose and poly(lactic‐ co ‐glycolic acid) on dendritic cell maturation
Author(s) -
Yoshida Mutsumi,
Babensee Julia E.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30798
Subject(s) - plga , biomaterial , materials science , agarose , microbiology and biotechnology , immune system , dendritic cell , cytokine , adjuvant , biophysics , in vitro , chemistry , biology , immunology , biochemistry , nanotechnology
Application of biomaterials in combination products in which the biomaterial is presented to the host with a biological component prompts the need for understanding the biomaterial‐associated adjuvant effect in the immune response against antigens associated with such a product. We have previously demonstrated that a polymer commonly used in tissue engineering and vaccine delivery, poly(lactic‐ co ‐glycolic acid) (PLGA), exerts an adjuvant effect in vivo , which was supported by PLGA‐induced dendritic cell (DC) maturation in vitro . In this study, the effects of agarose and PLGA on DC maturation were compared in vitro to establish differential biomaterial effects. Human monocyte‐derived DCs were treated with agarose or PLGA microparticles or films, and their maturation effect was measured as expression of costimulatory and MHC class II molecules, allostimulatory capacity, and proinflammatory cytokine secretion. Direct comparison of DC maturation phenotype indicated that PLGA was a stronger stimulus of DC maturation than agarose, and this maturation was not affected by microparticle phagocytosis. However, agarose‐treated DCs showed higher activation of nuclear factor κB (NFκB) 24 h after the initial stimulation of DCs. Taken together, these results demonstrate differential biomaterial effects on DC maturation, substantiating the maturation effect of PLGA, and provide screening methods for biomaterial adjuvant effect for applications in combination products. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006