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Cytokine release of mononuclear leukocytes (PBMC) after contact to a carbonated calcium phosphate bone cement
Author(s) -
Schildhauer T.A.,
Chapman J.R.,
Muhr G.,
Köller M.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30784
Subject(s) - peripheral blood mononuclear cell , concanavalin a , calcium , cytokine , receptor antagonist , materials science , immunology , microbiology and biotechnology , pharmacology , medicine , receptor , biology , antagonist , biochemistry , in vitro
Human leukocytes (peripheral blood mononuclear cells, PBMC) were overlaid on calcium phosphate bone cement (CBC, Norian SRS®) and allowed to settle for 1 h under cell culture conditions. Subsequently, the cells were either left unstimulated (i.e. sham stimulation using cell culture medium), or stimulated with toxic shock syndrome toxin‐1 (TSST‐1, 10 ng/mL), staphylococcal enterotoxin B (SEB, 10 ng/mL), or concanavalin A (ConA, 2 μg/mL) for further 24 h using cell culture conditions. Supernatants were then analyzed for cytokine content (interleukin‐1 receptor antagonist, IL‐1ra; IL‐2; IL‐6; IL‐10; IL‐12) by enzyme‐linked immunosorbent assay. While the spontaneous generation of cytokines was not influenced, the IL‐2 release from stimulated PBMC was significantly decreased in contrast to other analyzed cytokines after contact to the curing CBC compared to control incubations without CBC. This decrease in IL‐2 release was not due to known inhibitors of IL‐2 synthesis platelet factor‐4 (PF‐4), IL‐10, TGF‐β, or elevated calcium ion concentrations. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006