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Temperature‐induced cell detachment on immobilized pluronic surface
Author(s) -
Higuchi Akon,
Aoki Nobuo,
Yamamoto Taro,
Miyazaki Toyohiko,
Fukushima Hisashi,
Tak Tae Moon,
Jyujyoji Shin,
Egashira Satsuki,
Matsuoka Yuki,
Natori Shizue Hayashi
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30773
Subject(s) - poloxamer , laboratory flask , ethylene oxide , materials science , polystyrene , chromatography , chemical engineering , copolymer , chemistry , polymer , organic chemistry , composite material , engineering
The Pluronic F68 and F127, a triblock copolymer of ethylene oxide and propylene oxide, was activated using carbonyldiimidazole (CDI), and CDI‐activated Pluronic F68 and F127 was subsequently immobilized on the surface of a poly‐ L ‐lysine‐coated polystyrene tissue culture flask. Cell culture was performed on the Pluronic‐immobilized flask. The morphology of fibroblasts (L929 cells) on the Pluronic F127‐immobilized flask was mainly spherical, and showed less spreading behavior than that on the Pluronic F68‐immobilized flask and conventional tissue culture flask. This observation indicates that L929 cells on Pluronic F127‐immobilized flasks were cultured in a bio‐inert environment. L929 cells were successively detached from both Pluronic F127‐immobilized flask and Pluronic F68‐immobilized flask by cooling the flask to 4–15°C. This detachment is due to the hydration and dehydration properties of Pluronic, depending on the temperature. Umbilical cord blood was cultured in the Pluronic F127‐immobilized and conventional polystyrene tissue culture flasks at 37°C. The expression ratio of surface markers on hematopoietic stem cells (CD34 and CD133) cultured in the Pluronic F127‐immobilized flask was significantly higher than that of the cells in polystyrene tissue culture flask. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006