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Preparation, characterization and cytocompatibility of porous ACP/PLLA composites
Author(s) -
Gao Yanbo,
Weng Wenjian,
Cheng Kui,
Du Piyi,
Shen Ge,
Han Gaorong,
Guan Binggang,
Yan Weiqi
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30761
Subject(s) - materials science , composite number , apatite , porosity , amorphous calcium phosphate , composite material , simulated body fluid , phase (matter) , tissue engineering , phosphate , chemical engineering , calcium , biomedical engineering , scanning electron microscope , medicine , chemistry , organic chemistry , engineering , metallurgy
The purpose of this work was to incorporate amorphous calcium phosphate (ACP) into porous poly( L ‐lactic acid) (PLLA), because ACP is capable of fast phase transformation and morphological change in body fluid, such, a desired pore wall surface within bone tissue engineering scaffolds can be created. A highly porous ACP/PLLA composite was prepared by a thermally induced phase separation technique. The results showed that the composite had an interconnected pore structure with 100 μm macropores and 10 μm micropores, and 91% porosity; 40 nm primary particles of ACP were agglomerated to 3 μm aggregates, and the aggregates were homogeneously distributed in pore walls; These aggregates showed to be in situ transformed into bone‐like apatite after 1 h soaking in phosphate buffered saline solution. Human osteoblast‐like cell culture showed that the ACP/PLLA composite had better cell adhesion and alkaline phosphotase activity than pure PLLA. This study demonstrates that the ACP/PLLA composite can enhance cytocompatibility and could act as a promising scaffold for bone tissue engineering. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006