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Peritoneal application of chitosan and UV‐cross‐linkable chitosan
Author(s) -
Yeo Yoon,
Burdick Jason A.,
Highley Christopher B.,
Marini Robert,
Langer Robert,
Kohane Daniel S.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30740
Subject(s) - chitosan , materials science , composite material , chemical engineering , engineering
The suitability of chitosan and UV‐cross‐linkable chitosan for intraperitoneal use, for example as a barrier device for preventing peritoneal adhesions or for drug delivery, was examined. In vitro experiments using two major cell types present in the peritoneal cavity (mesothelial cells and peritoneal macrophages) revealed neither attractive interactions between cross‐linked chitosan gels and the cells nor a proliferative effect. However, the same UV‐cross‐linked chitosan applied in the peritoneal cavity of rabbits caused a granulomatous reaction with adhesion formation within two weeks in all animals, which persisted up to 4 weeks after exposure. Unmodified chitosan also caused adhesions, while UV irradiation did not. UV‐cross‐linkable chitosan induced significant elevations in MIP‐2 and TNF‐α from peritoneal macrophages, suggesting that soluble mediators could play a role in inducing adhesion formation. These results reinforce the view that the predictive value of in vitro cytotoxicity assays in matters of biocompatibility may not be sufficient, and suggest that other assays such as cytokine levels may be of value in predicting outcomes in situations involving multiple cell types (i.e. in vivo ). © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006