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The influence of the crystallinity of electrostatic spray deposition‐derived coatings on osteoblast‐like cell behavior, in vitro
Author(s) -
Siebers M.C.,
Walboomers X.F.,
Leeuwenburgh S.C.G.,
Wolke J.G.C.,
Jansen J.A.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30700
Subject(s) - crystallinity , materials science , osteoblast , chemical engineering , scanning electron microscope , titanium , simulated body fluid , apatite , coating , mineralization (soil science) , amorphous calcium phosphate , calcium , in vitro , composite material , metallurgy , chemistry , organic chemistry , biochemistry , nitrogen , engineering
This article describes the influence of the crystallinity of carbonate apatite (CA) coatings on osteoblast‐like cell behavior. Porous CA coatings were produced with electrostatic spray deposition (ESD), and subsequently, received heat treatments of 400, 500, or 700°C to induce various coating crystallinities. As a result, an amorphous calcium phosphate (ACP), a crystalline CA (CCA), and a crystalline carbonated hydroxyapatite (CHA) structure were formed, respectively. Uncoated titanium substrates served as the control group. After seeding rat osteoblast‐like cells, the initial cell attachment was similar between the groups, and approached 100% after 6 h. Between the various coatings, no differences were observed for proliferation, differentiation, or mineralization. However, proliferation of the osteoblast‐like cells was lower on all coated substrates after longer culture periods, compared to the uncoated substrates, while at the same time differentiation was stimulated. Furthermore, after 8 and 16 days of incubation, scanning electron microscopy showed more signs of mineralization on coated substrates, compared to the uncoated substrates. In conclusion, porous ESD‐derived CA coatings have a positive effect on the in vitro differentiation of osteoblast‐like cells, compared to uncoated, as‐machined titanium. However, this effect is not further enhanced by the degree of crystallinity of the ESD‐derived CA coatings. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006