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Soluble sustained release gene delivery system
Author(s) -
Bachrach Gilad,
Friedman Michael,
Gilinski Gladis,
Steinberg Doron
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30675
Subject(s) - materials science , gene delivery , plasmid , methacrylate , dissolution , polymer , genetic enhancement , dna , biophysics , dna supercoil , in vivo , nanotechnology , controlled release , copolymer , gene , chemical engineering , biochemistry , biology , genetics , dna replication , engineering , composite material
Delivery of genetic substance to target cells remains an obstacle for efficient utilization of gene therapy approaches. In this study, we describe a formulation of methacrylate acid copolymer carrier of DNA, in which the release rate of the gene can be controlled by pH. Plasmid release was coupled with the polymer's dissolution, which was accelerated in alkali conditions. The released plasmid was intact and bioactive, although alteration from closed circular supercoil to relaxed conformation was observed. Confocal laser scanning microscopy detected the plasmid DNA along the central layers of the polymeric film. Gene delivery systems controlled by the dissolution of the polymeric films offer flexibility in quantity and size of the incorporated DNA, and therefore could have a potential for in vivo use. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006