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Immobilized rhBMP‐2/succinylated type I atelocollagen gene expression of intracellular signaling molecules on ST2 cells
Author(s) -
Tsujigiwa Hidetsugu,
Nagatsuka Hitoshi,
Lee You Jin,
Han Phuu Pwint,
Gunduz Mehmet,
LeGeros Racquel Z.,
Inoue Masahisa,
Yamada Masao,
Nagai Noriyuki
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30661
Subject(s) - smad , intracellular , bone morphogenetic protein , signal transduction , microbiology and biotechnology , bone morphogenetic protein 2 , gene expression , biology , gene , biochemistry , in vitro
Recombinant human bone morphogenetic protein‐2 (rhBMP‐2) chemically‐bonded to succinylated type I atelocollagen, a biomaterial carrier with a porous structure, was reported to augment cellular activity of ST2 cells. The Smad protein family has been suggested to play an important role in the intracellular signaling pathway of BMP by its binding to receptors on target cells. However, there has been no study analyzing the downstream genes of the rhBMP‐2 induced intracellular signal transduction pathway. The purpose of this study was to examine the effect of immobilized rhBMP‐2 on gene expression of intracellular signaling molecules on ST2 cells. Our study showed two expression patterns of downstream genes of rhBMP‐2 intracellular signal transduction pathway. In the first pattern, BMPR‐IA , Smad 1 , and Smad 5 genes showed high basic expression before the addition of rhBMP, and the high level of gene expression continued for long period and decreased in the late stage when rhBMP‐2 was immobilized. In the second pattern, Smad 6 , Smad 7 , and Smad 8 genes showed low basic expression before the addition of rhBMP‐2 and a continuous increase from the beginning was followed by a decrease in the late stage when rhBMP‐2 was immobilized. Our results also showed that intracellular signaling continued for prolonged period when rhBMP‐2 was immobilized to succinylated type I atelocollagen. This study indicated that immobilizing rhBMP‐2 is an efficient method to increase bone induction. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006