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The effects of titanium and polymethylmethacrylate particles on osteoblast phenotypic stability
Author(s) -
Ramachandran R.,
Goodman S.B.,
Smith R.L.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30649
Subject(s) - materials science , osteolysis , osteocalcin , osteoblast , titanium , alkaline phosphatase , biomedical engineering , implant , biomaterial , biophysics , in vitro , nanotechnology , dentistry , metallurgy , biochemistry , surgery , medicine , chemistry , biology , enzyme
Wear particles generated following total joint arthroplasty interact with cells at the periprosthetic margin and induce an inflammatory response that contributes to osteolysis, aseptic loosening, and implant failure. This study examined the long‐term effects of particles from two commonly implanted materials, titanium (Ti) and polymethylmethacrylate (PMMA), on cell viability and metabolism over a 21‐day time course, using the human osteoblast‐like cell line MG‐63. Addition of particles was not associated with increased cell death or nitric oxide production at the particle concentration chosen. Collagen production was increased with exposure to titanium particles, whereas alkaline phosphatase and osteocalcin expression remained unchanged following exposure to both types of particles. The data show that titanium but not PMMA particles shifts bone cell metabolism to preferentially produce fibrous tissue rather than bone. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006