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Surface adsorption of DNA to tissue engineering scaffolds for efficient gene delivery
Author(s) -
Jang JaeHyung,
Bengali Zain,
Houchin Tiffany L.,
Shea Lonnie D.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30643
Subject(s) - materials science , adsorption , tissue engineering , gene delivery , dna , surface engineering , surface (topology) , nanotechnology , surface modification , chemical engineering , biomedical engineering , genetic enhancement , gene , organic chemistry , genetics , biology , medicine , chemistry , geometry , mathematics , engineering
Gene delivery from tissue engineering scaffolds has potential to promote localized transgene expression that can induce the formation of functional tissues. Substrate‐mediated delivery, an alternative delivery strategy to sustained release, is based on immobilization of DNA complexes to the polymer surface for subsequent delivery to cells cultured on the substrate. We investigate polyethylenimine (PEI)/DNA complex immobilization and subsequent cellular transfection on tissue engineering scaffolds fabricated from poly(lactide‐ co ‐glycolide) (PLG). The properties of the substrate and the complex affect both immobilization and cellular transfection. PLG promotes binding of PEI/DNA complexes, with percent bound independent of the N / P ratio or the DNA dosage. The levels of transgene expression are similar to or greater than control studies based on bolus DNA delivery, with orders of magnitude less DNA. Immobilization also homogeneously distributes the DNA throughout the scaffold, resulting in large numbers of transfected cells (>60%) at low surface quantities (<50 ng). Importantly, this approach can be employed to transfect cells throughout a three‐dimensional scaffold. Tissue engineering scaffolds that are prefabricated into various shapes from a range of materials could potentially employ this strategy for numerous applications. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006