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Modulating cell adhesion and spreading by control of FnIII 7–10 orientation on charged self‐assembled monolayers (SAMs) of alkanethiolates
Author(s) -
Wang Hua,
He Yi,
Ratner Buddy D.,
Jiang Shaoyi
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30586
Subject(s) - monolayer , biophysics , adhesion , materials science , adsorption , cell adhesion , cell , surface plasmon resonance , chemistry , nanotechnology , biochemistry , organic chemistry , composite material , nanoparticle , biology
In this work, we demonstrate that surface charge can be used to modulate cell adhesion/spreading through the control of the orientation of adsorbed FnIII 7–10 , which is a cell‐adhesive protein containing RGD residues. Carboxylic acid (COOH) and amine (NH 2 )‐terminated self‐assembled monolayers (SAMs) of alkanethiolates were used as model negatively and positively charged surfaces, respectively. The adsorbed amount of FnIII 7–10 is controlled to be equivalent on both SAMs as confirmed by the adsorption isotherms determined using I 125 ‐radiolabeled FnIII 7–10. The binding of a monoclonal antibody specific for the cell‐binding domain of FnIII 7–10 was measured by surface plasmon resonance (SPR) to evaluate FnIII 7–10 orientations on different SAMs. Results indicate that adsorbed FnIII 7–10 on NH 2 ‐SAM has an orientation with more cell‐binding domains accessible than on COOH‐SAM, confirming our predictions from Monte Carlo simulations. Both phase contrast images and Vybrant® MTT cell proliferation assays show that the adhesion/spreading of bovine aortic endothelial cells (BAECs) on the NH 2 ‐SAM is significantly better than that on the COOH‐SAM coated with an equivalent amount of FnIII 7–10 . These results indicate that surface charge can be used to specifically orient cell adhesive proteins such as FnIII 7–10 , thus providing a promising strategy to increase the activity of materials incorporating biological moieties. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006