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Autologous fibrin matrices: A potential source of biological mediators that modulate tendon cell activities
Author(s) -
Anitua Eduardo,
Sanchez Mikel,
Nurden Alan T,
Zalduendo Mar,
de la Fuente Maria,
Orive Gorka,
Azofra Juan,
Andia Isabel
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30585
Subject(s) - fibrin , tendon , regeneration (biology) , extracellular matrix , angiogenesis , neovascularization , wound healing , biomedical engineering , platelet , tissue engineering , matrix (chemical analysis) , microbiology and biotechnology , materials science , cancer research , immunology , pathology , medicine , biology , composite material
The use of autologous fibrin matrices has been proposed as a therapeutic strategy for the local and physiological delivery of growth factors in the treatment of several clinical conditions requiring tendon healing or tendon graft remodelling. In the present work, we investigated the proliferation, synthesis of type‐I collagen and angiogenic factors by tendon cells seeded on platelet‐rich (PR) and platelet‐poor (PP) matrices. Furthermore, in vivo cellular and vascular effects of each treatment were examined after infiltration in Achilles tendon in sheep. Results showed that the presence of platelets within the fibrin matrices increased significantly the proliferation of tendon cells. Additionally, cultured tendon cells synthesised type I collagen and angiogenic factors such as VEGF and HGF. The synthesis of VEGF, but not of HGF, was significantly higher when platelets were present within the matrix. In the sheep model, the injection of pre‐clotted plasma within tendons increased cellular density and promoted neovascularization. These results indicate that administration of fibrin matrices is a safe and easy strategy that may open new avenues for enhancing tissue healing and remodelling and influences the process of regeneration in clinical situations characterised by a poor healing outcome. © 2006 Wiley Periodicals, Inc. J Biomed Mater Res, 2006