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Surface modification and initial adhesion events for intestinal epithelial cells
Author(s) -
Hagerman E.M.,
Chao S.H.H.,
Dunn J.C.Y.,
Wu B.M.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30562
Subject(s) - fibronectin , microbiology and biotechnology , adhesion , biomaterial , materials science , cell adhesion , focal adhesion , cell adhesion molecule , tissue engineering , cell , integrin , surface modification , phosphorylation , biomedical engineering , nanotechnology , biology , extracellular matrix , chemistry , biochemistry , medicine , composite material
Rapid resealing of the mucosal epithelia is imperative following injuries to the small intestine because the mucosa is responsible for the adsorption of nutrients as well as providing a barrier to noxious agents present in the lumen. Tissue engineering may provide a possible solution for treating intestinal erosions, ulcerations, inflammatory bowel disease, and infection. Cell‐biomaterial interaction is a critical component in tissue engineering that can determine the success of the tissue construct. Cell‐biomaterial interactions can be enhanced by various types of surface modification, which promote integrin ligation leading to increased cell function. In order to relate the effect of surface adhesion molecules to signaling events and macroscopic cell response, an intestinal epithelial cell line, IEC‐6, was plated on fibronectin (receptor‐mediated) and poly‐ L ‐lysine (non‐specific) surfaces. Focal adhesion kinase (FAK) phosphorylation, cell spreading, and cell adhesion strength were measured. Results showed increases in FAK phosphorylation generally corresponded to increases in cell spreading and adhesion strength for IEC‐6 cells. Therefore, in a simplified system, initial adhesion and signaling mechanisms appeared to correspond to subsequent physical responses in IEC‐6 cells relevant to tissue engineering applications. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006