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Cellular response to phase‐separated blends of tyrosine‐derived polycarbonates
Author(s) -
Bailey LeeAnn O.,
Becker Matthew L.,
Stephens Jean S.,
Gallant Nathan D.,
Mahoney Christine M.,
Washburn Newell R.,
Rege Aarti,
Kohn Joachim,
Amis Eric J.
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30527
Subject(s) - materials science , fibronectin , extracellular matrix , annealing (glass) , downregulation and upregulation , biophysics , tyrosine , polymer , surface finish , chemical engineering , analytical chemistry (journal) , composite material , biochemistry , chromatography , biology , chemistry , gene , engineering
Two‐dimensional thin films consisting of homopolymer and discrete compositional blends of tyrosine‐derived polycarbonates were prepared and characterized in an effort to elucidate the nature of different cell responses that were measured in vitro . The structurally similar blends were found to phase separate after annealing with domain sizes dependent on the overall composition. The thin polymer films were characterized with the use of atomic force microscopy (AFM), water contact angles, and time‐of‐flight secondary ion mass spectrometry (TOF‐SIMS) and significant changes in roughness were measured following the annealing process. Genetic expression profiles of interleukin‐1β and fibronectin in MC3T3‐E1 osteoblasts and RAW 264.7 murine macrophages were measured at several time points, demonstrating the time and composition‐dependent nature of the cell responses. Real‐time reverse transcriptase polymerase chain reaction (RT‐PCR) depicted upregulation of the fibronectin gene copy numbers in each of the blends relative to the homopolymers. Moreover, the interleukin‐1β expression profile was found to be compositionally dependent. The data suggest strongly that optimal composition and processing conditions can significantly affect the acute inflammatory and extracellular matrix production responses. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006