Premium
Solution‐mediated effect of bioactive glass in poly (lactic‐ co ‐glycolic acid)–bioactive glass composites on osteogenesis of marrow stromal cells
Author(s) -
Yao Jun,
Radin Shula,
Reilly Gwendolen,
Leboy Phoebe S.,
Ducheyne Paul
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30494
Subject(s) - plga , glycolic acid , stromal cell , osteocalcin , materials science , bioactive glass , alkaline phosphatase , bone sialoprotein , scaffold , bone healing , bone marrow , biomedical engineering , mesenchymal stem cell , lactic acid , microbiology and biotechnology , cancer research , composite material , biochemistry , chemistry , nanotechnology , medicine , biology , immunology , surgery , enzyme , bacteria , nanoparticle , genetics
A previous study demonstrated that the incorporation of bioactive glass (BG) into poly (lactic‐ co ‐glycolic acid) (PLGA) can promote the osteoblastic differentiation of marrow stromal cells (MSCs) on PLGA by promoting the formation of a calcium–phosphate‐rich layer on its surface. To further understand the mechanisms underlying the osteogenic effect of PLGA‐BG composite scaffolds, whether solution‐mediated factors derived from composite scaffolds/hybrids can promote osteogenesis of marrow stromal cells was tested. The dissolution product from PLGA‐30%BG scaffold stimulated osteogenesis of MSCs, as was confirmed by increased mRNA expression of osteoblastic markers such as osteocalcin (OCN), alkaline phosphatase (ALP), and bone sialoprotein (BSP). The three‐dimensional structure of the scaffolds may contribute to the production of cell‐derived factors that promoted distant MSC differentiation. Thus PLGA‐BG composites demonstrate significant potential as a bone‐replacement material. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005