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Supramolecular assemblies of adsorbed collagen affect the adhesion of endothelial cells
Author(s) -
Keresztes Z.,
Rouxhet P. G.,
Remacle C.,
DupontGillain C.
Publication year - 2006
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30472
Subject(s) - materials science , adhesion , supramolecular chemistry , adsorption , affect (linguistics) , biophysics , cell adhesion , biomedical engineering , nanotechnology , chemical engineering , composite material , crystallography , organic chemistry , crystal structure , medicine , biology , chemistry , linguistics , philosophy , engineering
The behavior of endothelial cells (HUVECs) in contact with thin collagen films presenting different supramolecular organizations was investigated. Collagen was adsorbed on polystyrene (PS) and plasma‐oxidized PS (PSox) in conditions ensuring the formation of continuous layers presenting an increasing density of fibrillar structures. Discontinuous collagen layers were also prepared on PS by adsorption followed by dewetting. The morphology of the obtained collagen films was checked by using atomic force microscopy. HUVECs adhesion was evaluated in terms of cell number, cell area, cell shape, and actin structure after 4 h of contact with the prepared collagen layers. In the presence of serum, no adhesion was observed on PS, whereas a substantial adhesion was found on PSox. This is explained by the competition for adsorption, which turns in favor of adhesive proteins secreted by the cells on the hydrophilic PSox, but turns in favor of serum albumin on the hydrophobic PS. The progressive coating of PS by smooth collagen films increased cell adhesion and spreading. However, cell spreading and cytoskeleton organization were adversely affected by the appearance of a high density of collagen fibrillar structures. This latter trend was similarly observed on PSox. On the other hand, HUVECs spreading and cytoskeleton organization were clearly enhanced on discontinuous collagen layers compared with continuous ones. A possible explanation for these observations lies in the modification of exposure and/or spatial distribution of recognition sequences due to spontaneous collagen self‐assembly on fibril formation or to collagen aggregation on dewetting. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2006