Premium
Assimilating cell sheets and hybrid scaffolds for dermal tissue engineering
Author(s) -
Ng Kee Woei,
Tham Wanrong,
Lim Thiam Chye,
Werner Hutmacher Dietmar
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30454
Subject(s) - hyaluronic acid , extracellular matrix , glycolic acid , tissue engineering , materials science , biomedical engineering , dermis , cell , cell growth , cell culture , microbiology and biotechnology , biophysics , lactic acid , biochemistry , anatomy , chemistry , biology , medicine , genetics , bacteria
Cell sheets can be used to produce neo‐tissue with mature extracellular matrix. However, extensive contraction of cell sheets remains a problem. We devised a technique to overcome this problem and applied it to tissue engineer a dermal construct. Human dermal fibroblasts were cultured with poly(lactic‐ co ‐glycolic acid)‐collagen meshes and collagen‐hyaluronic acid foams. Resulting cell sheets were folded over the scaffolds to form dermal constructs. Human keratinocytes were cultured on these dermal constructs to assess their ability to support bilayered skin regeneration. Dermal constructs produced with collagen‐hyaluronic acid foams showed minimal contraction, while those with poly(lactic‐ co ‐glycolic acid)‐collagen meshes curled up. Cell proliferation and metabolic activity profiles were characterized with PicoGreen® and AlamarBlue™ assays, respectively. Fluorescent labeling showed high cell viability and F‐actin expression within the constructs. Collagen deposition was detected by immunocytochemistry and electron microscopy. Transforming Growth Factor‐α and β1, Keratinocyte Growth Factor and Vascular Endothelial Growth Factor were produced at various stages of culture, measured by RT‐PCR and ELISA. These results indicated that assimilating cell sheets with mechanically stable scaffolds could produce viable dermal‐like constructs that do not contract. Repeated enzymatic treatment cycles for cell expansion is unnecessary, while the issue of poor cell seeding efficiency in scaffolds is eliminated. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005