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Improved performance of primary rat hepatocytes on blended natural polymers
Author(s) -
Li Keguo,
Qu Xinjian,
Wang Yun,
Tang Yuefeng,
Qin Dajiang,
Wang Yanjun,
Feng Meifu
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30415
Subject(s) - hepatocyte , chitosan , gelatin , albumin , materials science , hepatocyte nuclear factor 4 , viability assay , cytoskeleton , hepatocyte growth factor , microbiology and biotechnology , chemical engineering , biochemistry , biophysics , cell , biology , in vitro , transcription factor , receptor , nuclear receptor , gene , engineering
Alginate (Alg), chitosan (Chi), collagen (Col), gelatin (Gel), and the mixtures of every two of them were screened to determine their suitability for hepatocyte culture. The test materials were fabricated as films and then evaluated on the basis of their abilities to promote the attachment and functions of rat hepatocytes cultured on them. Cellular attachment on Col and Gel was favorable. However, cellular viability, cytoskeleton organization and function, as evaluated by albumin production, ureagenesis, and enzyme activity of cytochrome P450 as well as expression levels of hepatocyte nuclear factor 4α deteriorated. Reverse cellular behavior was observed on Alg and Chi. Two blends, composed of Chi and Col or Gel, were found to be superior to other materials and sustained viability and differentiated functions of hepatocyte. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005