Premium
Transforming growth factor‐beta 1 (TGF‐β1) prevents the age‐dependent decrease in bone formation in human osteoblast/implant cultures
Author(s) -
Zhang Hai,
Aronow Michael S.,
Gronowicz Gloria A.
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30400
Subject(s) - osteoblast , bone sialoprotein , osteocalcin , alkaline phosphatase , implant , extracellular matrix , transforming growth factor , mineralization (soil science) , materials science , dentistry , medicine , endocrinology , in vitro , biology , microbiology and biotechnology , surgery , biochemistry , enzyme , ecology , soil water
Titanium implants have been extensively used in orthopedic surgery and dentistry. Most of the patients who receive such implants are elderly with a compromised ability to heal and form new bone. By using an in vitro osteoblast/implant culture system, the potency of TGF‐β1 in enhancing mineralization of human osteoblast cultures from elderly subjects was investigated in this study. Primary human osteoblast (HOB) cells obtained from different age group human subjects [Young (Y), Middle (M), and Old (O)] were cultured on Ti alloy (Ti‐6Al‐4V) disks with or without continuous administration of 0.2 ng/mL TGF‐β1 in the medium for 2 or 4 weeks. TGF‐β1 significantly ( p < 0.05) increased calcium content and the size of calcified nodules on implant disks in the O group, but had no effect on the Y or M groups. The number of calcified nodules was not different with or without TGF‐β1 in all age groups. As measured by Northern blot analysis and RT‐PCR, TGF‐β1 significantly increased the expression of bone‐specific extracellular matrix proteins, including alkaline phosphatase, Type I collagen, bone sialoprotein and osteocalcin, after both 2 and 4 weeks in the O group but not in the Y group. In conclusion, TGF‐β1 enhances mineralization on implant materials of osteoblast cultures from elderly human subjects. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005