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Collagen–carbon nanotube composite materials as scaffolds in tissue engineering
Author(s) -
MacDonald Rebecca A.,
Laurenzi Brendan F.,
Viswanathan Gunaranjan,
Ajayan Pulickel M.,
Stegemann Jan P.
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30386
Subject(s) - materials science , carbon nanotube , composite number , raman spectroscopy , tissue engineering , composite material , scanning electron microscope , matrix (chemical analysis) , chemical engineering , nanotechnology , biomedical engineering , medicine , physics , optics , engineering
Carbon nanotubes (CNT) are attractive for use in fiber‐reinforced composite materials due to their very high aspect ratio, combined with outstanding mechanical and electrical properties. Composite materials comprising a collagen matrix with embedded CNT were prepared by mixing solubilized Type I collagen with solutions of carboxylated single‐walled carbon nanotubes (SWNT) at concentrations of 0, 0.2, 0.4, 0.8, and 2.0 weight percent. Living smooth muscle cells were incorporated at the time of collagen gelation to produce cell‐seeded collagen–CNT composite matrices. Constructs containing 2.0 wt % CNT exhibited delayed gel compaction, relative to lower concentrations that compacted at the same rate as pure collagen controls. Cell viability in all constructs was consistently above 85% at both Day 3 and Day 7, whereas cell number in CNT‐containing constructs was lower than in control constructs at Day 3, though statistically unchanged by Day 7. Scanning electron microscopy showed physical interactions between CNT and collagen matrix. Raman spectroscopy confirmed the presence of CNT at the expected diameter (0.85–1.30 nm), but did not indicate strong molecular interactions between the collagen and CNT components. Such collagen–CNT composite matrices may have utility as scaffolds in tissue engineering, or as components of biosensors or other medical devices. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005