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Meniscal regeneration using tissue engineering with a scaffold derived from a rat meniscus and mesenchymal stromal cells derived from rat bone marrow
Author(s) -
Yamasaki Takuma,
Deie Masataka,
Shinomiya Rikuo,
Izuta Yasunori,
Yasunaga Yuji,
Yanada Shinobu,
Sharman Patrick,
Ochi Mitsuo
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30369
Subject(s) - meniscus , mesenchymal stem cell , fibrocartilage , tissue engineering , stromal cell , scaffold , materials science , bone marrow , extracellular matrix , biomedical engineering , regeneration (biology) , aggrecan , anatomy , pathology , microbiology and biotechnology , osteoarthritis , biology , medicine , physics , alternative medicine , incidence (geometry) , optics , articular cartilage
The purpose of this study was to regenerate a meniscus using a scaffold from a normal meniscus and mesenchymal stromal cells derived from bone marrow (BM‐MSCs). Thirty Sprague‐Dawley rat menisci were excised and freeze‐thawed three times with liquid nitrogen to kill the original meniscal cells. Bone marrow was aspirated from enhanced green fluorescent protein transgenic Sprague‐Dawley rats. BM‐MSCs were isolated, cultured for 2 weeks, and 2 × 10 5 cells were then seeded onto the meniscal scaffolds. Using a fluorescent microscope and immunohistochemical staining, repopulation of enhanced green fluorescent protein positive cells was observed in the superficial zone of the scaffold after 1 week of culture, and then in the deep zone after 2 weeks. At 4 weeks, expression of extracellular matrices was detected histologically and expression of mRNA for aggrecan and type X collagen was detected. Stiffness of the cultured tissue, assessed by the indentation stiffness test, had increased significantly after 2 weeks in culture, and approximated the stiffness of a normal meniscus. From this study, we conclude that a scaffold derived from a normal meniscus seeded with BM‐MSCs can form a meniscus approximating a normal meniscus. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005