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Evaluation of modifying collagen matrix with RGD peptide through periodate oxidation
Author(s) -
Zhang Lihai,
Hum Min,
Wang Meng,
Li Yanfeng,
Chen Hua,
Chu Chengbin,
Jiang Hua
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30363
Subject(s) - periodate , materials science , matrix (chemical analysis) , cell adhesion , adhesion , mesenchymal stem cell , scanning electron microscope , peptide , biophysics , biochemistry , chemistry , composite material , microbiology and biotechnology , biology
The aim of the study is to evaluate the effect of modifying collagen matrices with Arg‐Gly‐Asp (RGD) peptide through periodate oxidation. The collagen matrices were modified with RGD peptide, by periodate activation. The modified collagen matrices and unmodified matrices were characterized by scanning electron microscopy (SEM), differential scanning calorimetry (DSC), and electron spectroscopy for chemical analysis (ESCA). Mesenchymal stem cells (MSCs) were used to evaluate the cell compatibility of collagen matrices. In terms of cell growth, the MSCs attached much better on the modified matrix than on the unmodified one. But there was no significant difference between two groups regarding the MSC proliferation. Compared to the unmodified matrices, the mechanical strength of the modified matrix decreased sharply, and its 3D structure was destroyed. Introducing specific RGD receptor‐mediated adhesion sites on matrices obviously enhanced the MSC adhesion on collagen matrices, but the coupled method of periodate oxidation would likely result in the declination of the mechanical strength of the matrix, as well as the destruction of the matrix structure. This would affect the cell growth on the matrix, and decrease the histocompatibility of the matrices. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005