Premium
Culture of human umbilical vein endothelial cells on immobilized vascular endothelial growth factor
Author(s) -
Ito Yoshihiro,
Hasuda Hirokazu,
Terai Hiroshi,
Kitajima Takashi
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30360
Subject(s) - umbilical vein , materials science , vascular endothelial growth factor , human umbilical vein endothelial cell , endothelial stem cell , biomedical engineering , microbiology and biotechnology , vegf receptors , cancer research , biology , biochemistry , medicine , in vitro
Vascular endothelial growth factor (VEGF) was immobilized on substrata in photoreactive gelatin to control the adhesion and growth of vascular endothelial cells. The gelatin and VEGF were mixed in water and cast on a polystyrene dish or a silane‐coated glass plate. The surface was then photoirradiated in the presence or absence of a photomask and washed. Toughness of the immobilized material was confirmed by ethanol treatment. Human umbilical vein endothelial cells (HUVECs) grew on the immobilized VEGF but not on a nontreated surface. Growth of HUVEC increased significantly with an increase in the amount of immobilized VEGF, and the effects were inhibited by treatment with anti‐VEGF antibody. Thus, immobilized VEGF specifically interacted with HUVECs to permit growth in culture. Micropatterning of HUVEC cultures was also achieved using micropattern‐immobilized VEGF. This patterning technique may be useful for the formation of blood vessel networks in vitro . © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005