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Modulation of gene expression in neonatal rat cardiomyocytes by surface modification of polylactide‐ co ‐glycolide substrates
Author(s) -
Brown David A.,
Beygui Ramin E.,
MacLellan William R.,
Laks Hillel,
Dunn James C. Y.,
Wu Benjamin M.
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30344
Subject(s) - fibronectin , materials science , myosin , myocyte , microbiology and biotechnology , gene expression , biophysics , cell , substrate (aquarium) , biomedical engineering , gene , biology , biochemistry , medicine , ecology
Myocardial tissue engineering presents a potential treatment option for heart disease. Cardiomyocytes isolated at various stages of development retain the ability to form contractile networks in vitro, which suggests that it should be possible to reconstitute viable myocardium given the appropriate architecture, stimuli, and cardiomyogenic cell source. This study investigates the effects of modifying substrate surface energy (by plasma etching) and protein coating (by fibronectin adsorption) on neonatal rat ventricular myocyte (NRVM) function. Primary NRVMs were cultured for 96 h on modified and control films of a common degradable polymer, polylactide‐ co ‐glycolide. Cultures were analyzed for cell spreading, protein content, and mRNA expression of atrial natriuretic factor and β‐myosin heavy chain. The results demonstrate that NRVMs cultured on etched films significantly increased in spreading, myofibril development, protein content, and gene expression of atrial natriuretic factor and β‐myosin heavy chain compared with unetched films, and that this surface energy effect is overwhelmed by the addition of fibronectin. Conclusions from this study are that surface energy and protein adsorption influence the gene expression of adherent NRVMs, and may be important for modulating the function of engineered myocardium. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res, 2005