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Physical characteristics of small intestinal submucosa scaffolds are location‐dependent
Author(s) -
Raghavan Devanathan,
Kropp Bradley P.,
Lin HK.,
Zhang Yuanyuan,
Cowan Richard,
Madihally Sundararajan V.
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30268
Subject(s) - materials science , ultimate tensile strength , biomedical engineering , permeability (electromagnetism) , extracellular matrix , porosity , membrane , scanning electron microscope , submucosa , composite material , surgery , chemistry , medicine , biochemistry
Using biodegradable scaffolds as an alternative to engineer new tissues has become an attractive candidate in various transplantation protocols. In particular, small intestinal submucosa (SIS), a dense connective matrix harvested from the small intestine, has gained attention due to a number of favorable properties. However, use of SIS is constrained by obtaining reliable, reproducible products in large‐scale preparations that affect the regenerative process. To better understand the heterogeneous nature of SIS, this study focused on evaluating the location‐dependent alterations in the physical characteristics of the matrices harvested from distal and proximal ends and processed in‐house (referred as hand‐made). Additionally, results were compared with a commercially available machine‐made Cook® SIS. Tensile properties during monotonic loading and cyclical loading were compared in wet conditions. Furthermore, permeability of these membranes to urea was analyzed using a custom‐built chamber, and the microarchitecture was analyzed via scanning electron microscopy. These results showed that distal samples were more elastic and less permeable to urea relative to other samples. However, permeability in each sample was direction‐dependent, that is, mucosal to serosal direction was less permeable compared to sorasal to mucosal direction in all the samples. Cook® SIS was more susceptible to cyclical loading and had a shorter range of load carrying capacity. In summary, results show that physical characteristics of SIS are location‐dependent. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 90–96, 2005