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Regeneration of rat auditory ossicles using recombinant human BMP‐2/collagen composites
Author(s) -
Takeda Yasushi,
Tsujigiwa Hidetsugu,
Nagatsuka Hitoshi,
Nagai Noriyuki,
Yoshinobu Junko,
Okano Mitsuhiro,
Fukushima Kunihiro,
Takeuchi Ayako,
Yoshino Tadashi,
Nishizaki Kazunori
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30257
Subject(s) - ossicles , tympanoplasty , materials science , middle ear , ossification , tympanic cavity , biomedical engineering , regeneration (biology) , bone morphogenetic protein , anatomy , composite material , biology , medicine , surgery , microbiology and biotechnology , gene , biochemistry
Abstract Tympanoplasty operations to improve hearing impairment require a wide middle ear cavity and reconstruction of columellar formations. There is no specific material for use in the reconstruction of columellar formations. Tissue response to BMP has been employed as regenerative material. To our knowledge, however, there are no reports of the reconstruction of columellar formations using recombinant human bone morphogenetic protein‐2/bovine collagen composites in the middle ear. The purpose of this study is to investigate whether recombinant human bone morphogenetic protein‐2/bovine collagen composites (rhBMP‐2 composites) are appropriate for use as regenerative material for tympanoplasty. In the form of pellets, rhBMP‐2 composites were implanted as columellae into the tympanic cavity. At 2, 4, 6, 8, and 12 weeks after surgery, the middle ear of the animals (n = 4 at each week) was stained with hematoxylin‐eosin for light microscopic observation. All composites were in the process of ossification or had ossified according to their developmental stages and were covered with a single layer of squamous or cuboidal epithelium. The new bone formed in these composites was persistently stable and displayed some columellar conditions assessed by histological examination. This study led to the conclusion that rhBMP‐2 composites make excellent regenerative material for auditory ossicles. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 133–141, 2005