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Osteogenic potential of injectable tissue‐engineered bone: A comparison among autogenous bone, bone substitute (Bio‐Oss®), platelet‐rich plasma, and tissue‐engineered bone with respect to their mechanical properties and histological findings
Author(s) -
Ito Kenji,
Yamada Yoichi,
Nagasaka Tetsuro,
Baba Shunsuke,
Ueda Minoru
Publication year - 2005
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30248
Subject(s) - platelet rich plasma , trephine , materials science , biomedical engineering , tissue engineering , bone tissue , bone healing , mesenchymal stem cell , dentistry , medicine , platelet , pathology , anatomy
Recently, tissue engineering has become available as a regenerative treatment for bone defects. However, the evaluation of its success is limited to histological analysis, and its effects on mechanical hardness remain to be investigated. This study investigated mechanical strength in support of histological findings, specifically for tissue‐engineered bone with mesenchymal stem cells (MSCs) and platelet‐rich‐plasma (PRP). Initially, teeth were extracted, and bone defects on both sides of the mandible were prepared with a trephine bar. The defects were implanted by using the following graft materials: 1) PRP, 2) PRP and dog MSCs (dMSCs), 3) autogenous bone (PCBM), 4) bone substitute (Bio‐Oss®), and 5) control (defects only). After 2, 4, 8, and 12 weeks of implantation, the defects were histologically assessed to examine their mechanical properties. According to histological observations, the dMSCs/PRP groups had well‐formed mature bone compared with the control (defects only), Bio‐Oss®, and PRP groups. The Vickers hardness test values were 8 (control), 9 (PRP), not detected (Bio‐Oss®), 11 (PCBM), and 17 (dMSCs/PRP) after 2 weeks. Therefore, tissue‐engineered bone can be used for early stage bone regeneration from the viewpoint of histology and mechanical properties. © 2005 Wiley Periodicals, Inc. J Biomed Mater Res 73A: 63–72, 2005