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Human skin cell cultures onto PLA 50 (PDLLA) bioresorbable polymers: Influence of chemical and morphological surface modifications
Author(s) -
Garric Xavier,
Molès JeanPierre,
Garreau Henri,
Guilhou JeanJacques,
Vert Michel
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30216
Subject(s) - materials science , keratinocyte , lactic acid , glycolic acid , adhesion , fibroblast , cell adhesion , polymer , ethylene oxide , cell culture , polystyrene , biophysics , type i collagen , chemical engineering , in vitro , biochemistry , composite material , chemistry , biology , copolymer , bacteria , engineering , genetics , endocrinology
Poly(α‐hydroxy acid)s derived from lactic and glycolic acid are bioresorbable polymers which can cover a large range of thermal, physical, mechanical, and biological properties. Human keratinocytes have been shown as able to grow on a poly(DL‐lactic acid) film. However the keratinocyte growth was delayed with respect to culture on standard tissue culture polystyrene, even though the same plateau level was observed after 2 weeks. In order to improve the performance of poly(DL‐lactic acid) films as skin culture support, their surface was modified by creating tiny cavities using a method based on the leaching out of poly(ethylene oxide) from poly(lactic acid)–poly(ethylene oxide) heterogeneous blends. The surface of the films was also chemically modified by alkaline attack with sodium hydroxide and by type‐I collagen coating. Murine fibroblast cell line and primary cultures of human fibroblasts and of two types of keratinocytes were allowed to adhere and to grow comparatively on the different films. The presence of cavities affected neither the adhesion of dermal fibroblasts nor that of keratinocytes. Only keratinocyte proliferation was significantly reduced by the presence of cavities. Collagen coating improved skin cell adhesion and proliferation as well, except in the case of murine fibroblasts. In the case of the NaOH treatments, similar trends were observed but their extent depended on the treatment time. In the case of chemical modifications, fluorescence microscopy bore out adhesion and proliferation tendencies deduced from MTT tests. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 72A: 180–189, 2005