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Laminin–apatite composite coating to enhance cell adhesion to ethylene–vinyl alcohol copolymer
Author(s) -
Oyane Ayako,
Uchida Masaki,
Ito Atsuo
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30205
Subject(s) - apatite , materials science , composite number , copolymer , biocompatibility , composite material , coating , adhesion , chemical engineering , layer (electronics) , laminin , adsorption , polymer , chemistry , biochemistry , extracellular matrix , organic chemistry , metallurgy , engineering
A laminin–apatite composite layer with enhanced cell adhesive properties was successfully formed on the surface of an ethylene–vinyl alcohol copolymer (EVOH) by a liquid phase coating process. The coating process was carried out with the following procedure. First, an EVOH plate was alternately dipped in 200 m M calcium and 200 m M phosphate solutions to introduce nuclei or precursors of apatite to its surface. Second, the surface‐modified EVOH was immersed in a calcium phosphate solution, which was supersaturated with respect to apatite and containing laminin. As a result of this procedure, a laminin–apatite composite layer with a thickness of 2.5–3.0 μm was formed on the EVOH surface. Epithelial‐like cells (BSCC93) adhered to the laminin–apatite composite layer showed enhanced cell spreading, which was due to the biological effect of laminin. The number of cells adhered to the laminin–apatite composite layer on EVOH was approximately 10 times as large as that adhered to the surface of the untreated, laminin‐adsorbed, or apatite‐coated EVOH. Therefore, this type of composite material consisting of a synthetic polymer, apatite, and laminin has great potential as a skin terminal, with improved adhesiveness to skin tissue, as well as good biocompatibility. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 72A: 168–174, 2005