Premium
In vitro cytocompatibility assessment of β‐tricalcium phosphate/carboxymethyl‐chitin composite
Author(s) -
Muramatsu Kazuaki,
Nakajima Madoka,
Kikuchi Masanori,
Shimada Sawako,
Sasaki Kiyoshi,
Masuda Shingo,
Yoshihara Yusuke
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30197
Subject(s) - genotoxicity , chitin , fibroblast , materials science , ames test , fourier transform infrared spectroscopy , gel permeation chromatography , in vitro , carrageenan , nuclear chemistry , chitosan , chromatography , biochemistry , toxicity , chemistry , biology , organic chemistry , chemical engineering , polymer , composite material , salmonella , bacteria , engineering , genetics
A novel bioabsorbable bone substitute composed of a β‐tricalcium phosphate (β‐TCP) and a carboxymethyl‐chitin (CM‐chitin) sodium has been developed. Rabbit tibia defects (4 mm in diameter) were repaired after 4 weeks more effectively by the composite compared with a sham‐operation group. To further investigate the biological safety of the components, genotoxicity and carcinogenicity of an extract prepared from the composite were determined using four different in vitro assays. The main extract component was identified as CM‐chitin sodium [average molecular weight ( M w ) ∼230 kDa] as determined by Fourier transform infrared spectroscopy and gel permeation chromatography analysis. The concentrations of P and Ca possibly derived from β‐TCP were 17.7 and 37.1 μg/g, respectively, as determined by inductively coupled plasma mass spectroscopy. Both the metabolic activation and nonactivation (−S9) systems of the rat microsome S9 fraction were used to perform a genotoxicity evaluation using the Ames test and chromosome aberration assay on Chinese hamster lung fibroblast cells treated with the extract. In these assays, no genotoxicity was detected with doses ≤5 mg/mL (maximum concentration). The cell transformation assay using BALB/c 3T3 cells and the metabolic cooperation assay with V79 cells both showed negative results for any tumor‐promoting activity caused by the extract (∼5 mg/mL). These results indicate that the bioabsorbable β‐TCP/CM‐chitin composite is a highly biocompatible bone substitute. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 635–643, 2004