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Fibronectin adsorption on surface‐activated poly(dimethylsiloxane) and its effect on cellular function
Author(s) -
Toworfe George K.,
Composto Russell J.,
Adams Christopher S.,
Shapiro Irving M.,
Ducheyne Paul
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30164
Subject(s) - materials science , contact angle , x ray photoelectron spectroscopy , adsorption , substrate (aquarium) , chemical engineering , ellipsometry , polydimethylsiloxane , protein adsorption , fibronectin , polymer , polymer chemistry , thin film , composite material , nanotechnology , chemistry , cell , organic chemistry , oceanography , biochemistry , engineering , geology
This article reports that surface modification of poly(dimethylsiloxane) (PDMS) influences fibronectin (Fn) adsorption and enhances cell attachment. Controlled adsorption of Fn on chemically activated polymer substrates is known to influence cellular function. Thin films of PDMS were spun cast on silicon wafers to obtain homogeneous and molecularly smooth surfaces. The films were made hydrophilic by exposure to ultraviolet ozone activation (PDMS*). The films then were characterized by contact angle goniometry, ellipsometry, atomic force microscopy (AFM), Rutherford backscattering spectrometry and X‐ray photoelectron spectroscopy. Contact angle measurements indicated higher hydrophobicity of the nonactivated PDMS substrates than PDMS*. AFM scans of the substrates indicated higher surface roughness of PDMS* (Ra = 0.55 nm) than PDMS (Ra = 0.25 nm). Although Fn surface density (Γ) was slightly higher on PDMS than on PDMS*, due to hydrophobic interactions between substrate and Fn, cell function was greatly enhanced on the Fn‐coated PDMS* (PDMS*‐Fn) than on PDMS (PDMS‐Fn). Higher attachment of MC3T3‐E1 osteoblast‐like cells was observed on PDMS*‐Fn than on PDMS‐Fn. Moreover, cell spreading and cytoskeleton organization after 72 h was clearly favored on the Fn‐coated PDMS* surfaces. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 449–461, 2004