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The effect of different titanium and hydroxyapatite‐coated dental implant surfaces on phenotypic expression of human bone‐derived cells
Author(s) -
Knabe Christine,
Howlett Cameron Rolfe,
Klar Falk,
Zreiqat Hala
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30130
Subject(s) - bone sialoprotein , osteonectin , osteopontin , osseointegration , materials science , osteocalcin , titanium , osteoblast , alkaline phosphatase , implant , biomedical engineering , dental implant , biophysics , in vitro , chemistry , biochemistry , biology , metallurgy , immunology , medicine , surgery , enzyme
Roughened titanium (Ti) surfaces have been widely used for dental implants. In recent years, there has been the tendency to replace Ti plasma‐sprayed surfaces by sandblasted and acid‐etched surfaces in order to enhance osseous apposition. Another approach has been the utilization of hydroxyapatite (HA)‐coated implants. This study examines the effect of two roughened Ti dental implant surfaces on the osteoblastic phenotype of human bone–derived cells (HBDC) and compares this behavior to that for cells on an HA‐coated surface. Test materials were an acid‐etched and sandblasted Ti surface (Ti‐DPS), a porous Ti plasma‐sprayed coating (Ti‐TPS), and a plasma‐sprayed porous HA coating (HA). Smooth Ti machined surfaces served as control (Ti‐ma). HBDC were grown on the substrata for 3, 7, 14, and 21 days, counted and probed for various bone‐related mRNAs and proteins (type I collagen, osteocalcin, osteopontin, osteonectin, alkaline phosphatase, and bone sialoprotein). All dental implant surfaces significantly affected cellular growth and the temporal expression of an array of bone‐related genes and proteins. HA‐coated Ti had the most effect on osteoblastic differentiation inducing a greater expression of an array of osteogenic markers than recorded for cells grown on Ti‐DPS and Ti‐TPS, thus suggesting that the HA‐coated surface may possess a higher potency to enhance osteogenesis. Furthermore, Ti‐DPS surfaces induced greater osteoblast proliferation and differentiation than Ti‐TPS. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 71A: 98–107, 2004