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A new bone‐inducing biodegradable porous β‐tricalcium phosphate
Author(s) -
Matsushita Naofumi,
Terai Hidetomi,
Okada Takao,
Nozaki Kazutoshi,
Inoue Hikaru,
Miyamoto Shimpei,
Takaoka Kunio
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30102
Subject(s) - biomaterial , materials science , polyethylene glycol , biomedical engineering , biodegradable polymer , polymer , chemistry , biochemistry , nanotechnology , medicine , composite material
A new type of degradable biomaterial with bone‐inducing capacity was made by combining porous β‐tricalcium phosphate (β‐TCP) with a delivery system for recombinant human bone morphogenetic protein‐2 (rhBMP‐2). The BMP delivery system consisted of a block copolymer composed of poly‐ D , L ‐lactic acid with random insertion of p ‐dioxanone and polyethylene glycol (PLA‐DX‐PEG), a known biocompatible and biodegradable material. The efficacy of this biomaterial in terms of its bone‐inducing capacity was examined by ectopic bone formation in the dorsal muscles of the mouse. In the β‐TCP implants coated with the PLA‐DX‐PEG polymer containing more than 0.0025% (w/w) of rhBMP‐2, new ectopic bone tissues with marrow were consistently found on the surface of implants. The radiographic density of β‐TCP was diminished in a time‐dependent manner. On histological examination, numerous multinucleated osteoclasts with positive tartrate‐resistant acid‐phosphatase (TRAP) staining were noted on the surface of the β‐TCP. These experimental results indicate that β‐TCP implants coated with synthetic rhBMP‐2 delivery system might provide effective artificial bone‐graft substitutes with osteoinductive capacity and biodegradable properties. In addition, this type of biomaterial may require less rhBMP‐2 to induce significant new bone mass. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 450–458, 2004