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Hyaluronic acid grafting mitigates calcification of glutaraldehyde‐fixed bovine pericardium
Author(s) -
Ohri Rachit,
Hahn Sei K.,
Hoffman Allan S.,
Stayton Patrick S.,
Giachelli Cecilia M.
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30088
Subject(s) - glutaraldehyde , calcification , hyaluronic acid , osteopontin , grafting , materials science , chemistry , anatomy , pathology , medicine , chromatography , immunology , organic chemistry , polymer
Pathologic calcification is the leading cause of the clinical failure of glutaraldehyde‐fixed bovine pericardium used in bioprosthetic valves. A novel surface modification of glutaraldehyde fixed bovine pericardium was carried out with high molecular weight hyaluronic acid (HA). HA was chemically modified with adipic dihydrazide (ADH) to introduce hydrazide functional groups onto the HA backbone. Glutaraldehyde‐fixed bovine pericardium (GFBP) was modified by grafting this HA to the free aldehyde groups on the tissue via the hydrazide groups. Following a 2‐week subcutaneous implantation in osteopontin (OPN)‐null mice, the calcification of HA‐modified bovine pericardium was drastically reduced (by 84.5%) compared to positive controls (tissue without HA‐modification) ( p = 0.005). The calcification‐mitigating effect of HA surface modification was also confirmed by microscopic analysis of explanted tissue stained with Alizarin Red S for calcium. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 328–334, 2004