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Differential expression of osteogenic factors associated with osteoinductivity of human osteosarcoma cell lines
Author(s) -
Yu Yan,
Harris Richard I.,
Yang JiaLin,
Anderson H. Clarke,
Walsh William R.
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30072
Subject(s) - smad , osteosarcoma , in vivo , bone morphogenetic protein , bone morphogenetic protein 2 , transforming growth factor beta , cell culture , cancer research , in vitro , mesenchymal stem cell , transforming growth factor , cell , microbiology and biotechnology , chemistry , medicine , biology , gene , biochemistry , genetics
Differential expression of multiple osteogenic factors may be responsible for the different osteoinductivity of osteosarcoma cell lines. We compared in vivo osteoinductivity of human osteosarcoma cell lines (Saos‐2 vs. U‐2 OS) in nude mice, and their in vitro expression of various osteogenic factors of protein level by quantitative immunocytochemistry and mRNA level by RT‐PCR and/or in situ hybridization. Saos‐2 cells, but not U‐2 OS, were osteoinductive in vivo . Significantly higher expression (independent t ‐test, all p < 0.005) of osteogenic factors were observed in Saos‐2 cells compared with U‐2 OS, which included bone morphogenetic proteins (particularly BMPs‐2, 3, 4, and 7), transforming growth factor‐beta (TGF‐β), BMP receptor (BMPR)‐1A, receptor‐regulated Smads (R‐Smads), Smads 1, 2, and 5, and common‐mediator Smad (Co‐Smad), Smad 4. In contrast, U‐2 OS cells expressed higher levels of inhibitory Smad 6 (I‐Smad) protein than Saos‐2 cells ( p < 0.001). These results suggest that a combination of osteogenic factors (BMPs, TGF‐β, BMPRs, and R/Co‐Smads) against I‐Smad may play important roles in the Saos‐2 cell osteoinductivity. This may have a clinical implication in selecting key osteogenic factors for combined therapy for bone defect diseases. The characterized cell lines can be used as positive and negative controls for the assessments of both in vitro and in vivo bone formation capabilities of designed tissues or biomaterials. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 70A: 122–128, 2004