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Inhibition of macrophage development and foreign body giant cell formation by hydrophilic interpenetrating polymer network
Author(s) -
Collier Terry O.,
Anderson James M.,
Brodbeck William G.,
Barber Thomas,
Healy Kevin E.
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.30030
Subject(s) - foreign body giant cell , adhesion , biomaterial , cell adhesion , macrophage , materials science , monocyte , protein adsorption , giant cell , biophysics , microbiology and biotechnology , ethylene glycol , chemistry , polymer , immunology , nanotechnology , in vitro , biology , biochemistry , composite material , medicine , pathology , genetics , organic chemistry
The ability of monocytes to adhere, differentiate into macrophages, and fuse to form foreign body giant cells (FBGCs) on an implanted material surface is a critical step toward biomaterial degradation. Novel homogenous surfaces were utilized to mediate adhesion. These surfaces consisted of N ‐(2 aminoethyl)‐3‐aminopropyltrimethoxysilane (EDS) and an interpenetrating polymer network (IPN) of polyacrylamide and poly(ethylene glycol). These surfaces were designed to control cell adhesion and morphology and mediate cell differentiation, activation, metabolic ability, and apoptosis, resulting in a reduced or controlled inflammatory response. The EDS surface promotes cell adhesion and the IPN minimizes protein adsorption and subsequent cell adhesion. Both surfaces had similar cellular adhesion rates at each respective time point. However, the adherent macrophage morphology was similar at 2 h and day 3, and at days 7 and 10 adherent macrophages on the EDS surface formed FBGCs (46% at day 7 and 40% at day 10). Adherent cells on the IPN surface did not form FBGCs but instead formed monocyte aggregates (73% of adherent cells formed aggregates at day 7 and 63% at day 10). It is indicated that the two surface chemistries differentially controlled monocyte differentiation into macrophages and subsequent macrophage fusion to form FBGCs. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 644–650, 2004