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Micro‐Raman spectroscopic investigation of dental calcified tissues
Author(s) -
Schulze K. A.,
Balooch M.,
Balooch G.,
Marshall G. W.,
Marshall S. J.
Publication year - 2004
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.20130
Subject(s) - dentin , materials science , cementum , raman spectroscopy , enamel paint , mineralized tissues , nanoindentation , analytical chemistry (journal) , nuclear magnetic resonance , mineralogy , composite material , optics , chemistry , physics , chromatography
The purpose of this study was to determine if dental calcified junctions (DEJs/CDJs) in human teeth contain different compositional phases compared to the adjacent dental calcified tissues. Peak positions and intensities were determined from micro‐Raman spectra for PO 3− 4and the CH modes and compared among the mineralized tissues and their junctions. Values of width were determined from the intersections of intensity regression lines through the junctions and in the adjacent tissues. The peaks were measured in 1‐μm steps along a l00‐μm line across the junction. High‐resolution analysis revealed that PO 3− 4band peaks for dentin, the DEJ, enamel, the CDJ, and cementum were at the same position (959 cm −1 ), while for the CH stretching mode a significant shift of 4.6 cm −1 was found between enamel, the DEJ, and dentin. The mean width of the DEJ was 7.6 (± 2.8) μm using the PO 3− 4band and 8.6 (± 3.6) μm using the CH stretching mode. Across the DEJ, the mineral content monotonically decreased from enamel to dentin while the organic component monotonically increased. The DEJ width was in agreement with prior nanoindentation studies. No width estimate was possible for the CDJ because the compositional differences between cementum and dentin were small. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 286–293, 2004