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Competitive plasma protein adsorption onto fluorinated polyimide surfaces
Author(s) -
Kawakami H.,
Kanno M.,
Nagaoka S.,
Kubota S.
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.20052
Subject(s) - polyimide , adsorption , materials science , protein adsorption , biocompatibility , scanning electron microscope , curing (chemistry) , chemical engineering , blood proteins , adhesion , polymer chemistry , composite material , polymer , organic chemistry , layer (electronics) , chemistry , biochemistry , engineering , metallurgy
A series of fluorinated polyimides cured at different temperatures was prepared, and plasma protein adsorption and platelet adhesion onto the polyimide films were evaluated in vitro using scanning electron microscopy, a micro‐bicinchoninic acid protein assay, and a gold–colloid‐labeled immunoassay. In particular, we focused on competitive plasma protein adsorption onto polyimide film because elucidation of the competitive adsorption mechanism is needed for a good understanding of in vivo biocompatibility of polyimide. Interestingly, the trend of IgG adsorption onto the polyimide surface measured in human plasma was completely contrary to that observed with IgG dissolved in PBS, and the adsorption increased with an increase in the curing temperature. We propose that the human plasma F c region in IgG might selectively adsorb onto polyimide film cured at high temperatures because of competitive plasma protein adsorption to the surface. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 1393–1400, 2003