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Peripheral blood cell separation through surface‐modified polyurethane membranes
Author(s) -
Higuchi Akon,
Yamamiya Shinichi,
Yoon Boo Ok,
Sakurai Masaru,
Hara Mariko
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.20005
Subject(s) - membrane , platelet , permeation , cd34 , materials science , blood cell , haematopoiesis , red blood cell , stem cell , biophysics , chemistry , biology , biochemistry , microbiology and biotechnology , immunology
Cell separation from peripheral blood was investigated using surface‐modified polyurethane (PU) membranes with different functional groups. Both red blood cells and platelets could pass through unmodified PU and PU‐SO 3 H membranes, whereas the red blood cells preferentially passed through PU‐N(C 2 H 5 ) 2 and PU‐NHC 2 H 4 OH membranes. The permeation ratio of T and B cells was <25% for the surface‐modified and unmodified PU membranes. CD34 + cells have been recognized as various kinds of stem cells including hematopoietic and mesenchymal stem cells. The adhesiveness of CD34 + cells on the PU membranes was found to be higher than that of red blood cells, platelets, T cells, or B cells. Overall, the adhesiveness of blood cells on the PU membranes increased in the following order: red blood cells ≤ platelets < T cells ≤ B cells < CD34 + cells. Treatment of PU‐COOH membranes with a human albumin solution to detach adhered blood cells, allowed recovery of mainly CD34 + cells in the permeate, whereas both red blood cells and platelets could be isolated in the permeate using unmodified PU membranes. The PU membranes showed different permeation and recovery ratios of specific cells depending on the functional groups attached to the membranes. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 68A: 34–42, 2004