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TEGDMA‐induced toxicity in human fibroblasts is associated with early and drastic glutathione depletion with subsequent production of oxygen reactive species
Author(s) -
Stanislawski Lena,
Lefeuvre Mathieu,
Bourd Katia,
SoheiliMajd Esmat,
Goldberg Michel,
Périanin Axel
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10600
Subject(s) - trolox , glutathione , cytotoxicity , reactive oxygen species , dichlorofluorescein , toxicity , oxidative stress , buthionine sulfoximine , antioxidant , materials science , ascorbic acid , chemistry , biochemistry , biophysics , pharmacology , in vitro , biology , organic chemistry , food science , antioxidant capacity , enzyme
Triethylene glycol dimethacrylate (TEGDMA) is a dentin‐bonding agent and a major component of various dental restorative biomaterials. TEGDMA monomers are released from dental resins and induce dental pulp inflammation and necrosis. In this study, we have investigated the mechanism of TEGDMA–induced cytotoxicity of fibroblasts. Treatment of cultured human gingival and pulpal fibroblasts with 0.1–3 m M of TEGDMA for 24 h induced a concentration–dependent and variable cytotoxic effect. Fifty percent of toxicity (TC 50 ) was obtained with 1.2 ± 0.9 and 2.6 ± 1.1 m M of TEGDMA for gingival and pulpal fibroblasts, respectively. Moreover, TEGDMA‐induced cytotoxicity was associated with an early and drastic depletion of cellular glutathione (GSH), which started at 15–30 min and was almost complete at 4–6 h. Antioxidants, such as Trolox (0.01 m M ), ascorbate (0.2 m M ), and N‐acetylcysteine (NAC) (5 m M ) prevented the TEGDMA‐induced cytotoxicity while GSH depletion was partially inhibited. Finally, a late production of reactive oxygen species (ROS) occurred in fibroblasts treated with TEGDMA for 3–4 h, as determined by 2′,7′‐dichlorofluorescein fluorescence, and was completely inhibited by Trolox (5 μ M ). The data show that TEGDMA induced a drastic GSH depletion followed by production of ROS, which may contribute to the toxicity of gingival and pulpal fibroblasts. Antioxidants, such as NAC, ascorbate, and particularly Trolox, appear useful in preventing cell damage mediated by resin‐containing dental restorative materials. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 66A: 476–482, 2003