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Preparation and culture of hepatocyte on gelatin microcarriers
Author(s) -
Tao Xu,
Shaolin Li,
Yaoting Yu
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10277
Subject(s) - microcarrier , gelatin , hepatocyte , materials science , chemical engineering , biophysics , cell , in vitro , chemistry , biochemistry , biology , engineering
Porous gelatin microcarriers having a diameter of 80–100 μm were prepared by the suspension method using toluene as the oil phase. Rat hepatocytes were cultured on gelatin and cytodexIII microcarriers. The cells retained its spherical shape, which is similar in vivo , and showed no morphological changes to the flat state. Hepatocyte aggregates on microcarriers maintained higher metabolic functions than monolayer cells. Pore size of microcarrier plays an important role in the attachment and metabolic function of cells in culture. Phase‐contrast micrograph and cell activity showed that hepatocytes cultures on gelatin microcarrier of <1 μm pore size is better than that of 5–10 μm. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 306–310, 2003