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Regeneration of serous membrane on gelatin‐processed polyglycolic acid (PGA)‐human collagen membrane and its efficacy on the prevention of adhesion
Author(s) -
Lee Yong Ho,
Nakamura Tatsuo,
Shimizu Yasuhiko,
Yamamoto Yasumich,
Kiyotani Tetsuya,
Tsuda Touru,
Teramachi Masayoshi,
Takimoto Yukinobu
Publication year - 2002
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10199
Subject(s) - gelatin , adhesion , membrane , connective tissue , tissue adhesion , cecum , materials science , regeneration (biology) , serous membrane , biomedical engineering , medicine , pathology , chemistry , microbiology and biotechnology , biology , biochemistry , composite material
We prepared a complex membrane consisting of human amnion‐derived collagen membrane and polyglycolic acid (PGA), and then gelatin was crosslinked using heat on one side of the complex membrane to prepare a membrane that can prevent adhesion (gelatin‐processed PGA–human collagen membrane). Applying this membrane to a rabbit cecum‐abdominal‐wall‐adhesion model, the prevention of adhesion and tissue regeneration were investigated. The animals were sacrificed 2 and 12 weeks after surgery and then examined. The adhesion scores in the short‐term observation group (2 weeks after surgery) and long‐term observation group (12 weeks after surgery) were 1.0 ± 2.4 and 0.8 ± 2.0, respectively, showing a significant prevention of adhesion compared to the control value of 6.3 ± 2.5 ( p < 0.01). Histologically, gelatin was not absorbed, and outgrowth of connective tissue accompanied by capillary blood vessels was observed between the sample and the cecum in the short‐term observation group. In the long‐term observation group, the sample was completely absorbed, and serous membrane was regenerated on the surface of connective tissue. Based on these findings, it is possible to use gelatin‐processed PGA–human collagen membrane as a filling material with both an adhesion‐preventing effect and tissue‐regenerating function. © 2002 Wiley Periodicals, Inc. J Biomed Mater Res 64A: 88–92, 2003