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Surface‐modified poly(lactide‐ co ‐glycolide) nanospheres for targeted bone imaging with enhanced labeling and delivery of radioisotope
Author(s) -
Park Yoon Jeong,
Nah Sook Hee,
Lee Jue Yeon,
Jeong Jae Min,
Chung Jun Key,
Lee Myung Chul,
Yang Victor C.,
Lee Seung Jin
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10167
Subject(s) - biodistribution , materials science , plga , poloxamer , lactide , surface modification , ethylene oxide , biomedical engineering , nuclear chemistry , in vivo , chemical engineering , nanoparticle , copolymer , nanotechnology , chemistry , polymer , medicine , composite material , engineering , microbiology and biotechnology , biology
Surface‐modified nanospheres can be utilized for targeting drugs and diagnostic agents to the bone and bone marrow while extending their circulation time in the blood stream. The surface modification of poly(lactide‐ co ‐glycolide) (PLGA) nanospheres by radioisotope carrying poly(ethylene oxide)‐poly (propylene oxide)‐poly (ethylene oxide) triblock copolymers (Poloxamer 407) has been assessed by in vitro characterization and in vivo biodistribution studies after intravenous administration of the nanospheres to the mouse. A hydroxyphenylpropionic acid, a ligand for 125 I and 131 I labeling, was conjugated to the hydroxyl group of the Poloxamer 407 by using dicyclohexyl carbodiimide. The ligand‐conjugated Poloxamer 407 was adsorbed onto the surface of PLGA nanospheres. Surface coating was confirmed by measuring both size distribution and the surface charge of the nanospheres. Besides, 125 I‐labeling efficiency, radiolabeling stability, whole body imaging, and biodistribution of the radioisotope‐labeled nanospheres were examined. Ligand‐labeled, surface‐modified PLGA nanospheres were in 100‐nm size ranges, which may be adequate for long‐circulation and further bone imaging. 125 I‐labeling efficiency was >90% and was more stable at human serum for 24 h. A noticeable decrease in liver or spleen uptake was obtained by the surface‐modified nanospheres. 125 I‐labeled nanospheres showed higher blood maintenance and bone uptake compared with stannous colloid with the same size distribution. Therefore, a fully biodegradable, radioisotope‐carrying, surface‐modified nanosphere system has been developed as a promising tool for targeting bone and bone marrows. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 751–760, 2003