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Direct attachment of fibronectin to tresyl chloride‐activated titanium
Author(s) -
Hayakawa Tohru,
Yoshinari Masao,
Nemoto Kimiya
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10143
Subject(s) - titanium , chloride , fibronectin , materials science , x ray photoelectron spectroscopy , fourier transform infrared spectroscopy , inorganic chemistry , chemical engineering , chemistry , metallurgy , extracellular matrix , biochemistry , engineering
The aim of this study was to bind fibronectin directly to a titanium surface treated with tresyl chloride (2,2,2‐trifluoroethanesulfonyl chloride) for the development of a strong connection of a dental implant to subepithelial connective tissues and/or peri‐implant epithelia. Basic terminal OH groups of mirror polished titanium were allowed to react with tresyl chloride at 37°C for 2 days. The tresylated titanium disk was then immersed into a fibronectin/phosphate‐buffered saline solution for 24 h at 37°C. The activation reaction of the basic OH of titanium with tresyl chloride was confirmed by S2p, F1s, and O1s spectra using X‐ray photoelectron spectroscopy and OSO 2 bonds using Fourier transform infrared reflection‐absorption spectroscopy. After the reaction of fibronectin with titanium, the X‐ray photoelectron spectroscopy revealed the remarkable effect of the activation of terminal OH groups with the tresyl chloride treatment. The N1s peak derived from the attached fibronectin still remained after 60 s of argon‐ion sputtering after tresyl chloride treatment. In contrast, the N1s peak of the specimen not treated with tresyl chloride almost disappeared after only 10 s of argon‐ion etching. Fibronectin, a well‐known cell‐adhesive protein, could easily be attached to the titanium surface by use of the tresyl chloride activation technique. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 684–688, 2003