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Interactions of antithrombin and proteins in the plasma contact activation system with immobilized functional heparin
Author(s) -
Cornelius Rena M.,
Sanchez Javier,
Olsson Per,
Brash John L.
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10118
Subject(s) - factor xii , adsorption , antithrombin , heparin , chromatography , sodium dodecyl sulfate , prekallikrein , elution , gel electrophoresis , materials science , polyacrylamide gel electrophoresis , chemistry , biophysics , coagulation , kallikrein , biochemistry , organic chemistry , enzyme , biology , medicine
The interactions of antithrombin (AT) and the contact phase clotting factors with two commercially available heparinized surfaces are reported. The Carmeda (CBAS) and Corline surfaces along with controls (a sulfonated polyethylene surface and a CBAS analog in which the heparin used was devoid of specific AT‐binding sequences) were exposed to human plasma. Adsorbed proteins were eluted and examined by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis and immunoblotting. The CBAS and Corline surfaces adsorbed large amounts of AT, whereas adsorption on the controls was negligible. Immunoblots for the four contact phase clotting factors indicated less contact activation on the CBAS and Corline surfaces than on the controls. Determination of adsorbed functional AT using a FXa inhibition assay showed that the CBAS surface adsorbed about 4 times as much AT as the Corline surface. Adsorption of AT to the control surfaces was minimal. Assays for adsorbed FXII and FXIIa based on kallikrein generation showed that all four surfaces adsorbed similar amounts of FXII. However, on the controls, most of the FXII was in activated form, whereas on the CBAS and Corline surfaces very little activation occurred. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 475–483, 2003