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Novel method of preparing acellular cardiovascular grafts by decellularization with poly(ethylene glycol)
Author(s) -
Uchimura Eiichiro,
Sawa Yoshiki,
Taketani Satoshi,
Yamanaka Yuka,
Hara Masayuki,
Matsuda Hikaru,
Miyake Jun
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10097
Subject(s) - ethylene glycol , decellularization , materials science , extracellular matrix , amphiphile , aqueous solution , polymer , tissue engineering , copolymer , biomedical engineering , biochemistry , chemistry , organic chemistry , composite material , medicine
We have developed a new method of preparing acellular vascular grafts. Cellular components, including cell membranes and proteins in cytosol, were efficiently extracted from the vessels in a concentrated aqueous solution of poly(ethylene glycol), an amphiphilic biocompatible polymer. The residual DNA was digested by deoxyribonuclease I treatment after extraction with poly(ethylene glycol). The two‐step extraction process proved quite effective at removing the cellular components while causing little damage to the extracellular matrices. We did not use any detergent that would damage the extracellular matrices. Therefore, vascular endothelial cells grew well on the acellular vessels after recellularization, promising longi‐patent cardiovascular grafts. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 834–837, 2003