Premium
In vitro release kinetics of proteins from bioactive foams
Author(s) -
Lenza R. F. S.,
Jones J. R.,
Vasconcelos W. L.,
Hench L. L.
Publication year - 2003
Publication title -
journal of biomedical materials research part a
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.849
H-Index - 150
eISSN - 1552-4965
pISSN - 1549-3296
DOI - 10.1002/jbm.a.10042
Subject(s) - simulated body fluid , materials science , covalent bond , dissolution , kinetics , apatite , tissue engineering , chemical engineering , in vitro , ternary operation , bioactive glass , biophysics , chemistry , biomedical engineering , biochemistry , organic chemistry , composite material , medicine , physics , quantum mechanics , engineering , biology , computer science , programming language
This study describes an approach to obtaining 3‐D scaffolds for tissue engineering that allows the incorporation and release of biologically active proteins to stimulate cell function. Laminin was adsorbed on the textured surfaces of binary 70S30C (70 mol % SiO 2 , 30 mol % CaO) and ternary 58S (60 mol % SiO 2 , 36 mol % CaO, 4 mol % P 2 O 5 ) foams. The covalent bonds between the binding sites of the proteins and the ligands on the scaffolds' surfaces did not denaturate the proteins. In vitro studies show that the foams modified with chemical groups and coated with laminin were bioactive, as demonstrated by the formation of a crystalline hydroxy carbonate apatite (HCA) layer formed on the surfaces of the foams upon exposure to simulated body fluid (SBF). The release of proteins from the foams also was investigated. Sustained and controlled release from the scaffolds over a 30‐day period was achieved. Laminin release from the bioactive foams followed the dissolution rate of the material network. These results suggest that bioactive foams have the potential to act as scaffolds for soft‐tissue engineering with a controlled release of proteins that can induce tissue formation or regeneration. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 121–129, 2003