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Author(s) -
Heckl Christian,
Lang Alexander,
Rühm Adrian,
Sroka Ronald,
Duffield Thomas,
Vogeser Michael,
Paal Michael
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Reports
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202170016
Subject(s) - methemoglobin , hemolysis , pigment , cover (algebra) , front cover , hemoglobin , chemistry , heme , chromatography , medicine , biochemistry , organic chemistry , engineering , mechanical engineering , enzyme
Standard methods inadequately address the presence of methemoglobin and methemalbumin that are indicative of severe intravascular hemolysis with rapid hemoglobin release. Here we present a novel second derivative fitting approach evaluating UV/VIS spectra for simultaneous quantitative analysis of oxyhemoglobin, methemoglobin, methemalbumin, and bilirubin, providing added diagnostic value when compared to standard spectrophotometric methods that typically underestimate the total free heme pigment content. Further details can be found in the article by Christian Heckl, Alexander Lang, Adrian Rühm, Ronald Sroka, Thomas Duffield, Michael Vogeser, Michael Paal ( e202000461 )