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Raman confocal microscopy and biophysics multiparametric characterization of the skin barrier evolution with age
Author(s) -
Rigal Aline,
MichaelJubeli Rime,
Nkengne Alex,
BailletGuffroy Arlette,
Bigouret Armelle,
Tfayli Ali
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202100107
Subject(s) - stratum corneum , epidermis (zoology) , biophysics , raman spectroscopy , chemistry , microscopy , transepidermal water loss , confocal microscopy , skin aging , thickening , confocal , skin barrier , bound water , anatomy , biology , pathology , molecule , microbiology and biotechnology , dermatology , medicine , optics , physics , organic chemistry , polymer science
Abstract Skin aging is a multifactorial phenomenon that involves alterations at the molecular, cellular and tissue levels. Our aim was to carry out a multiparametric biophysical and Raman characterization of skin barrier between individuals of different age groups (<24 and >70 years old). Our results showed a significant decrease of lipids to proteins ratio overall the thickness of the stratum corneum and higher lateral packing in the outer part of the SC for elderly. This can explain the decrease in trans epidermal water loss measured values rather than only SC thickening. Both age groups showed similar water content at SC surface while elderly presented higher water content in deep SC and viable epidermis. Mechanical measurements showed a decrease in the elasticity and an increase in the fatigability with age and were correlated with partially bound water. Highest correlation and anti‐correlation values were observed for the deepest part of the SC and the viable epidermis.