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High ‐speed volumetric imaging in vivo based on structured illumination microscopy with interleaved reconstruction
Author(s) -
Shi Ruheng,
Li Yuting,
Kong Lingjie
Publication year - 2021
Publication title -
journal of biophotonics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.877
H-Index - 66
eISSN - 1864-0648
pISSN - 1864-063X
DOI - 10.1002/jbio.202000513
Subject(s) - microscopy , lossless compression , computer science , light sheet fluorescence microscopy , fluorescence microscope , focus (optics) , artificial intelligence , computer vision , iterative reconstruction , depth of field , optical microscope , optics , biomedical engineering , physics , fluorescence , scanning confocal electron microscopy , engineering , scanning electron microscope , data compression
Wide‐field fluorescence microscopy (WFFM) is widely adopted in biomedical studies, due to its high imaging speed over large field‐of‐views. However, WFFM is susceptible to out‐of‐focus background. To overcome this problem, structured illumination microscopy (SIM) was proposed as a wide‐field, optical‐sectioning technique, which needs multiple raw images for image reconstruction and thus has a lower imaging speed. Here we propose SIM with interleaved reconstruction, to make SIM of lossless speed. We apply this method in volumetric imaging of neural network dynamics in brains of zebrafish larva in vivo.